› 3pqj

X-ray diffraction
2.48Å resolution

Crystal Structure of the transcriptional repressor BigR from Xylella fastidiosa

Released:
DOI: 10.2210/pdb3pqj/pdb
PDB entry 3pqj coloured by chain, front view.
PDB entry 3pqj coloured by chain, side view.
PDB entry 3pqj coloured by chain, top view.
Source organism: Xylella fastidiosa
Primary publication:
Plant pathogenic bacteria utilize biofilm growth-associated repressor (BigR), a novel winged-helix redox switch, to control hydrogen sulfide detoxification under hypoxia.
Guimarães BG, Barbosa RL, Soprano AS, Campos BM, de Souza TA, Tonoli CC, Leme AF, Murakami MT, Benedetti CE
J Biol Chem 286 26148-57 (2011)
PMID: 21632538

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biofilm growth-associated repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 102 amino acids
Theoretical weight: 11.52 KDa
Source organism: Xylella fastidiosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PFB1 (Residues: 13-114; Coverage: 90%)
Gene names: XF_0767, bigR
Sequence domains: Bacterial regulatory protein, arsR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P1
Unit cell:
a: 40.59Å b: 47.84Å c: 54.7Å
α: 90.03° β: 89.96° γ: 105.34°
R-values:
R R work R free
0.197 0.194 0.25
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Mechanisms and regulation of surface interactions and biofilm formation in Agrobacterium.
Heindl et al. (2014)
9 more

5 mentions without citation

Plant pathogenic bacteria utilize biofilm growth-associated repressor (BigR), a novel winged-helix redox switch, to control hydrogen sulfide detoxification under hypoxia.
Guimarães et al. (2011)
4 more