› 3psv

X-ray diffraction
2Å resolution

Structure of E97D mutant of TIM from Plasmodium falciparum

Released:
DOI: 10.2210/pdb3psv/pdb
PDB entry 3psv coloured by chain, front view.
PDB entry 3psv coloured by chain, side view.
PDB entry 3psv coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Revisiting the mechanism of the triosephosphate isomerase reaction: the role of the fully conserved glutamic acid 97 residue.
Samanta M, Murthy MR, Balaram H, Balaram P
Chembiochem 12 1886-96 (2011)
PMID: 21671330

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 27.98 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07412 (Residues: 1-248; Coverage: 100%)
Gene name: TPI
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR-H
Unit cell:
a: 51.061Å b: 53.767Å c: 174.698Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.185 0.229
Expression system: Escherichia coli

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Citations

1 review citation

Enzyme architecture: on the importance of being in a protein cage.
Richard et al. (2014)
9 other articles