› 3pwx

X-ray diffraction
2.5Å resolution

Structure of putative flagellar hook-associated protein from Vibrio parahaemolyticus

Released:
DOI: 10.2210/pdb3pwx/pdb
PDB entry 3pwx coloured by chain, front view.
PDB entry 3pwx coloured by chain, side view.
PDB entry 3pwx coloured by chain, top view.
Source organism: Vibrio parahaemolyticus
Entry authors: Ramagopal UA, Patskovsky Y, Toro R, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flagellin N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 26.29 KDa
Source organism: Vibrio parahaemolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q87JH9 (Residues: 39-268; Coverage: 77%)
Gene name: VPA0274
Sequence domains: Bacterial flagellin N-terminal helical region
Structure domains: f41 fragment of flagellin, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 100.687Å b: 63.509Å c: 80.57Å
α: 90° β: 107.86° γ: 90°
R-values:
R R work R free
0.234 0.23 0.294
Expression system: Escherichia coli BL21(DE3)

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