› 3q19

X-ray diffraction
1.9Å resolution

Human Glutathione Transferase O2

Released:
DOI: 10.2210/pdb3q19/pdb
PDB entry 3q19 coloured by chain, front view.
PDB entry 3q19 coloured by chain, side view.
PDB entry 3q19 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the dehydroascorbate reductase activity of human omega-class glutathione transferases.
Zhou H, Brock J, Liu D, Board PG, Oakley AJ
J Mol Biol 420 190-203 (2012)
PMID: 22522127

Function and Biology Details

Reactions catalysed: 
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H(2)O
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase omega-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H4Y5 (Residues: 1-239; Coverage: 98%)
Gene name: GSTO2
Sequence domains: Glutathione S-transferase, N-terminal domain
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 46.425Å b: 85.017Å c: 60.397Å
α: 90° β: 94.55° γ: 90°
R-values:
R R work R free
0.165 0.164 0.194
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Human genetic variation influences vitamin C homeostasis by altering vitamin C transport and antioxidant enzyme function.
Michels et al. (2013)
3 more

4 mentions without citation

The still mysterious roles of cysteine-containing glutathione transferases in plants.
Lallement et al. (2014)
3 more