› 3q8g

X-ray diffraction
1.8Å resolution

Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution

Released:
DOI: 10.2210/pdb3q8g/pdb
PDB entry 3q8g coloured by chain, front view.
PDB entry 3q8g coloured by chain, side view.
PDB entry 3q8g coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution.
Schaaf G, Dynowski M, Mousley CJ, Shah SD, Yuan P, Winklbauer EM, de Campos MK, Trettin K, Quinones MC, Smirnova TI, Yanagisawa LL, Ortlund EA, Bankaitis VA
OpenAccess logo Mol Biol Cell 22 892-905 (2011)
PMID: 21248202

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRAL-TRIO domain-containing protein YKL091C Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 37.36 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33324 (Residues: 1-310; Coverage: 100%)
Gene name: YKL091C
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PEE 1 x PEE
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 49.503Å b: 71.388Å c: 99.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.229
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Phosphatidylinositol transfer proteins and instructive regulation of lipid kinase biology.
Grabon et al. (2015)
9 more

2 mentions without citation

Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach.
Ramu et al. (2016)
1 more