› 3qku

X-ray diffraction
3.3Å resolution

Mre11 Rad50 binding domain in complex with Rad50 and AMP-PNP

Released:
DOI: 10.2210/pdb3qku/pdb
PDB entry 3qku coloured by chain, front view.
PDB entry 3qku coloured by chain, side view.
PDB entry 3qku coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair.
Williams GJ, Williams RS, Williams JS, Moncalian G, Arvai AS, Limbo O, Guenther G, SilDas S, Hammel M, Russell P, Tainer JA
OpenAccess logo Nat Struct Mol Biol 18 423-31 (2011)
PMID: 21441914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-157667 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA double-strand break repair Rad50 ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 41.06 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P58301 (Residues: 1-187, 194-882; Coverage: 40%)
Gene names: PF1167, rad50
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
DNA double-strand break repair protein Mre11 Chain: C
Molecule details ›
Chain: C
Length: 34 amino acids
Theoretical weight: 4.06 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1N9 (Residues: 348-381; Coverage: 8%)
Gene names: PF1166, mre11

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P6522
Unit cell:
a: 177.398Å b: 177.398Å c: 130.423Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.258 0.308
Expression system: Escherichia coli

Quick links

Citations

46 review citations

Double-strand break end resection and repair pathway choice.
Symington et al. (2011)
45 more

10 mentions without citation

Archaeal genome guardians give insights into eukaryotic DNA replication and damage response proteins.
Shin et al. (2014)
9 more