› 3qle

X-ray diffraction
1.83Å resolution

Structural Basis for the Function of Tim50 in the Mitochondrial Presequence Translocase

Released:
DOI: 10.2210/pdb3qle/pdb
PDB entry 3qle coloured by chain, front view.
PDB entry 3qle coloured by chain, side view.
PDB entry 3qle coloured by chain, top view.
Source organism: Saccharomyces cerevisiae EC1118
Primary publication:
Structural basis for the function of Tim50 in the mitochondrial presequence translocase.
Qian X, Gebert M, Höpker J, Yan M, Li J, Wiedemann N, van der Laan M, Pfanner N, Sha B
J Mol Biol 411 513-9 (2011)
PMID: 21704637

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial import inner membrane translocase subunit TIM50 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.89 KDa
Source organism: Saccharomyces cerevisiae EC1118
Expression system: Escherichia coli
UniProt:
  • Canonical: C8ZIW7 (Residues: 164-361; Coverage: 42%)
Gene name: EC1118_1P2_2410g
Sequence domains: NLI interacting factor-like phosphatase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ACT 1 x ACT
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 84.109Å b: 84.109Å c: 116.549Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.175 0.202
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Mitochondrial protein import: common principles and physiological networks.
Dudek et al. (2013)
10 more

7 mentions without citation

Interactions of amyloidogenic proteins with mitochondrial protein import machinery in aging-related neurodegenerative diseases.
Reed et al. (2023)
6 more