› 3qwf

X-ray diffraction
1.88Å resolution

Crystal structure of the 17beta-hydroxysteroid dehydrogenase from Cochliobolus lunatus

Released:
DOI: 10.2210/pdb3qwf/pdb
PDB entry 3qwf coloured by chain, front view.
PDB entry 3qwf coloured by chain, side view.
PDB entry 3qwf coloured by chain, top view.
Source organism: Curvularia lunata
Primary publication:
Insights into subtle conformational differences in the substrate-binding loop of fungal 17β-hydroxysteroid dehydrogenase: a combined structural and kinetic approach.
Cassetta A, Krastanova I, Kristan K, Brunskole Švegelj M, Lamba D, Lanišnik Rižner T, Stojan J
Biochem J 441 151-60 (2012)
PMID: 21929506

Function and Biology Details

Reaction catalysed: 
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17beta-hydroxysteroid dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 270 amino acids
Theoretical weight: 28.94 KDa
Source organism: Curvularia lunata
Expression system: Escherichia coli
UniProt:
  • Canonical: O93874 (Residues: 1-270; Coverage: 100%)
Gene name: 17HSDcl
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 8 x NAP
1 bound ligand:
Ligand GOL 10 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P1
Unit cell:
a: 65.52Å b: 67.83Å c: 138.89Å
α: 84.2° β: 85.31° γ: 66.57°
R-values:
R R work R free
0.167 0.165 0.194
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Aerobic iron-based cross-dehydrogenative coupling enables efficient diversity-oriented synthesis of coumestrol-based selective estrogen receptor modulators.
Kshirsagar et al. (2013)
3 more