› 3r38

X-ray diffraction
2.23Å resolution

2.23 Angstrom resolution crystal structure of UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) from Listeria monocytogenes EGD-e

Released:
DOI: 10.2210/pdb3r38/pdb
PDB entry 3r38 coloured by chain, front view.
PDB entry 3r38 coloured by chain, side view.
PDB entry 3r38 coloured by chain, top view.
Source organism: Listeria monocytogenes
Entry authors: Halavaty AS, Minasov G, Shuvalova L, Dubrovska I, Winsor J, Peterson S, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 48.77 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8Y4C4 (Residues: 1-430; Coverage: 100%)
Gene names: lmo2526, murA, murA1
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 144.579Å b: 51.923Å c: 78.178Å
α: 90° β: 110.95° γ: 90°
R-values:
R R work R free
0.198 0.196 0.239
Expression system: Escherichia coli BL21

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