› 3rdo

X-ray diffraction
1.4Å resolution

Crystal structure of R7-2 streptavidin complexed with biotin

Released:
DOI: 10.2210/pdb3rdo/pdb
PDB entry 3rdo coloured by chain, front view.
PDB entry 3rdo coloured by chain, side view.
PDB entry 3rdo coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding.
Magalhães ML, Czekster CM, Guan R, Malashkevich VN, Almo SC, Levy M
Protein Sci 20 1145-54 (2011)
PMID: 21520321

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 15.97 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 37-164; Coverage: 81%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

4 bound ligands:
Ligand BTN 1 x BTN
Ligand NI 1 x NI
Ligand SO4 1 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4122
Unit cell:
a: 57.36Å b: 57.36Å c: 184.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.171
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Functional protein nanostructures: a chemical toolbox.
Kuan et al. (2018)
12 other articles