› 3rdx

X-ray diffraction
2.1Å resolution

Crystal structure of ligand-free R7-2 streptavidin

Released:
DOI: 10.2210/pdb3rdx/pdb
PDB entry 3rdx coloured by chain, front view.
PDB entry 3rdx coloured by chain, side view.
PDB entry 3rdx coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding.
Magalhães ML, Czekster CM, Guan R, Malashkevich VN, Almo SC, Levy M
Protein Sci 20 1145-54 (2011)
PMID: 21520321

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-149691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 15.97 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 37-164; Coverage: 81%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 79.25Å b: 81.64Å c: 84.537Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Functional protein nanostructures: a chemical toolbox.
Kuan et al. (2018)
12 other articles

1 mention without citation

Femtosecond X-ray diffraction from two-dimensional protein crystals.
Frank et al. (2014)