› 3ri5

X-ray diffraction
3.4Å resolution

C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab, ivermectin and picrotoxin

Released:
DOI: 10.2210/pdb3ri5/pdb
Model geometry
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PDB entry 3ri5 coloured by chain, front view.
PDB entry 3ri5 coloured by chain, side view.
PDB entry 3ri5 coloured by chain, top view.
Source organisms:
Primary publication:
Principles of activation and permeation in an anion-selective Cys-loop receptor.
Hibbs RE, Gouaux E
Nature 474 54-60 (2011)
PMID: 21572436

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-211641 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Avermectin-sensitive glutamate-gated chloride channel GluCl alpha Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 347 amino acids
Theoretical weight: 39.64 KDa
Source organism: Caenorhabditis elegans
Expression system: Spodoptera frugiperda
Structure domains:
Mouse monoclonal Fab fragment, heavy chain Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 221 amino acids
Theoretical weight: 23.92 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Mouse monoclonal Fab fragment, light chain Chains: K, L, M, N, O

Ligands and Environments

7 bound ligands:
Ligand IVM 5 x IVM
Ligand LMT 3 x LMT
Ligand NAG 3 x NAG
Ligand OCT 3 x OCT
Ligand UND 1 x UND
Ligand CL 1 x CL
Ligand RI5 1 x RI5
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 154.843Å b: 154.843Å c: 573.473Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.232 0.248
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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Citations

131 review citations

Structure, function, and modulation of GABA(A) receptors.
Sigel et al. (2012)
130 more

11 mentions without citation

The recombinant expression systems for structure determination of eukaryotic membrane proteins.
He et al. (2014)
10 more

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