› 3riu

X-ray diffraction
3.4Å resolution

Crystal structure of Drosophila hexameric C3PO formed by truncated Translin and Trax

Released:
DOI: 10.2210/pdb3riu/pdb
PDB entry 3riu coloured by chain, front view.
PDB entry 3riu coloured by chain, side view.
PDB entry 3riu coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Multimeric assembly and biochemical characterization of the Trax-translin endonuclease complex.
Tian Y, Simanshu DK, Ascano M, Diaz-Avalos R, Park AY, Juranek SA, Rice WJ, Yin Q, Robinson CV, Tuschl T, Patel DJ
OpenAccess logo Nat Struct Mol Biol 18 658-64 (2011)
PMID: 21552261

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181521 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Translin Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 25.32 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7JVK6 (Residues: 1-217; Coverage: 92%)
Gene names: CG11761, Dmel\CG11761, Dmel_CG11761, TB-RBP, translin, trsn
Sequence domains: Translin family
Structure domains:
Translin-associated protein X Chain: C
Molecule details ›
Chain: C
Length: 269 amino acids
Theoretical weight: 31.47 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VF77 (Residues: 30-298; Coverage: 90%)
Gene names: CG5063, Dmel\CG5063, Dmel_CG5063, TRAX, Trax, Trax-RB, trax
Sequence domains: Translin family
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6122
Unit cell:
a: 196.03Å b: 196.03Å c: 155.189Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.274 0.27 0.338
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Molecular mechanisms of RNA interference.
Wilson et al. (2013)
17 more

2 mentions without citation

Multimeric assembly and biochemical characterization of the Trax-translin endonuclease complex.
Tian et al. (2011)
1 more