› 3rj6

X-ray diffraction
1.23Å resolution

Crystal Structure of Horse heart ferric myoglobin; K45E/K63E/K96E mutant

Released:
DOI: 10.2210/pdb3rj6/pdb
PDB entry 3rj6 coloured by chain, front view.
PDB entry 3rj6 coloured by chain, side view.
PDB entry 3rj6 coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Charge-Disproportionation Symmetry Breaking Creates a Heterodimeric Myoglobin Complex with Enhanced Affinity and Rapid Intracomplex Electron Transfer.
Trana EN, Nocek JM, Woude JV, Span I, Smith SM, Rosenzweig AC, Hoffman BM
J Am Chem Soc 138 12615-28 (2016)
PMID: 27646786

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 16.98 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68082 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 28.341Å b: 123.565Å c: 34.986Å
α: 90° β: 89.73° γ: 90°
R-values:
R R work R free
0.174 0.173 0.197
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Design of artificial metalloproteins/metalloenzymes by tuning noncovalent interactions.
Hirota et al. (2018)
2 other articles

3 mentions without citation

BODIPY-Based Fluorescent Probes for Sensing Protein Surface-Hydrophobicity.
Dorh et al. (2015)
2 more