› 3rkp

X-ray diffraction
2.24Å resolution

Crystal structure of BcpA*(D312A), the major pilin subunit of Bacillus cereus

Released:
DOI: 10.2210/pdb3rkp/pdb
PDB entry 3rkp coloured by chain, front view.
PDB entry 3rkp coloured by chain, side view.
PDB entry 3rkp coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Isopeptide bonds of the major pilin protein BcpA influence pilus structure and bundle formation on the surface of Bacillus cereus.
Hendrickx AP, Poor CB, Jureller JE, Budzik JM, He C, Schneewind O
Mol Microbiol 85 152-63 (2012)
PMID: 22624947

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 355 amino acids
Theoretical weight: 39.23 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81D71 (Residues: 163-515; Coverage: 67%)
Gene name: BC_2508
Sequence domains: Prealbumin-like fold domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 68.862Å b: 68.096Å c: 97.251Å
α: 90° β: 107.73° γ: 90°
R-values:
R R work R free
0.213 0.21 0.255
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The cell wall architecture of Enterococcus faecium: from resistance to pathogenesis.
Hendrickx et al. (2013)
3 more

3 mentions without citation

Isopeptide bonds of the major pilin protein BcpA influence pilus structure and bundle formation on the surface of Bacillus cereus.
Hendrickx et al. (2012)
2 more