› 3rof

X-ray diffraction
1.03Å resolution

Crystal Structure of the S. aureus Protein Tyrosine Phosphatase PtpA

Released:
DOI: 10.2210/pdb3rof/pdb
PDB entry 3rof coloured by chain, front view.
PDB entry 3rof coloured by chain, side view.
PDB entry 3rof coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structure and substrate recognition of the Staphylococcus aureus protein tyrosine phosphatase PtpA.
Vega C, Chou S, Engel K, Harrell ME, Rajagopal L, Grundner C
J Mol Biol 413 24-31 (2011)
PMID: 21871460

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143026 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Low molecular weight protein-tyrosine-phosphatase PtpA Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.95 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C5D2 (Residues: 1-152; Coverage: 99%)
Gene name: ptpA
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold
Expression tag cleaved from protein-tyrosine-phosphatase ptpA Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 853 Da
Source organism: Staphylococcus aureus
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 34.81Å b: 35.1Å c: 65.51Å
α: 90° β: 91.05° γ: 90°
R-values:
R R work R free
0.15 0.15 0.169
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Protein tyrosine phosphatase structure-function relationships in regulation and pathogenesis.
Böhmer et al. (2013)
2 more

4 mentions without citation

Structure and substrate recognition of the Staphylococcus aureus protein tyrosine phosphatase PtpA.
Vega et al. (2011)
3 more