› 3rr2

X-ray diffraction
1.95Å resolution

Structure of a Cysteine synthase (O-Acetylserine Sulfhydrylase (OASS)) from Mycobacterium marinum ATCC BAA-535 / M

Released:
DOI: 10.2210/pdb3rr2/pdb
PDB entry 3rr2 coloured by chain, front view.
PDB entry 3rr2 coloured by chain, side view.
PDB entry 3rr2 coloured by chain, top view.
Source organism: Mycobacterium marinum M
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-109576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 32.8 KDa
Source organism: Mycobacterium marinum M
Expression system: Escherichia coli
UniProt:
  • Canonical: B2HL85 (Residues: 1-310; Coverage: 100%)
Gene names: MMAR_3645, cysK1
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P43212
Unit cell:
a: 51.19Å b: 51.19Å c: 229.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.232
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more

1 mention without citation

Insights into multifaceted activities of CysK for therapeutic interventions.
Joshi et al. (2019)