› 3rso

X-ray diffraction
1.6Å resolution

H-Ras soaked in 20% S,R,S-bisfuranol: 1 of 10 in MSCS set

Released:
DOI: 10.2210/pdb3rso/pdb
PDB entry 3rso coloured by chain, front view.
PDB entry 3rso coloured by chain, side view.
PDB entry 3rso coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Analysis of binding site hot spots on the surface of Ras GTPase.
Buhrman G, O'Connor C, Zerbe B, Kearney BM, Napoleon R, Kovrigina EA, Vajda S, Kozakov D, Kovrigin EL, Mattos C
J Mol Biol 413 773-89 (2011)
PMID: 21945529

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GNP 1 x GNP
Ligand CA 2 x CA
Ligand MG 1 x MG
Ligand RSG 2 x RSG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R32
Unit cell:
a: 88.496Å b: 88.496Å c: 134.094Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.198
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Drugging the undruggable RAS: Mission possible?
Cox et al. (2014)
19 more

5 mentions without citation

Analysis of binding site hot spots on the surface of Ras GTPase.
Buhrman et al. (2011)
4 more