› 3s4g

X-ray diffraction
6Å resolution

Low Resolution Structure of STNV complexed with RNA

Released:
DOI: 10.2210/pdb3s4g/pdb
PDB entry 3s4g coloured by chain, front view.
PDB entry 3s4g coloured by chain, side view.
PDB entry 3s4g coloured by chain, top view.
Source organisms:
Primary publication:
Construction and crystal structure of recombinant STNV capsids.
Lane SW, Dennis CA, Lane CL, Trinh CH, Rizkallah PJ, Stockley PG, Phillips SE
J Mol Biol 413 41-50 (2011)
PMID: 21839089

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 180-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137130 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Capsid protein Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 21.74 KDa
Source organism: Satellite tobacco necrosis virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03606 (Residues: 1-196; Coverage: 100%)
Sequence domains: Satellite tobacco necrosis virus coat protein
RNA (5'-R(P*AP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 3 nucleotides
Theoretical weight: 943 Da
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(P*UP*UP*UP*U)-3') Chain: C
Molecule details ›
Chain: C
Length: 4 nucleotides
Theoretical weight: 1.18 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2
Unit cell:
a: 313.146Å b: 302.121Å c: 183.385Å
α: 90° β: 93.82° γ: 90°
R-values:
R R work R free
0.38 not available 0.43
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Construction and characterization of virus-like particles: a review.
Zeltins A. (2013)
3 more

4 mentions without citation

Nucleic acid packaging in viruses.
Speir et al. (2012)
3 more