› 3sb1

X-ray diffraction
1.67Å resolution

Hydrogenase expression protein HupH from Thiobacillus denitrificans ATCC 25259

Released:
DOI: 10.2210/pdb3sb1/pdb
PDB entry 3sb1 coloured by chain, front view.
PDB entry 3sb1 coloured by chain, side view.
PDB entry 3sb1 coloured by chain, top view.
Source organism: Thiobacillus denitrificans ATCC 25259
Entry authors: Filippova EV, Minasov G, Shuvalova L, Kiryukhina O, Ruan J, Leo RD, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HupH hydrogenase expression protein C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 160 amino acids
Theoretical weight: 17.34 KDa
Source organism: Thiobacillus denitrificans ATCC 25259
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3SJ38 (Residues: 1-136; Coverage: 100%)
Gene name: Tbd_1379
Sequence domains: HupH hydrogenase expression protein, C-terminal conserved region
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 83.306Å b: 39.291Å c: 65.192Å
α: 90° β: 104.74° γ: 90°
R-values:
R R work R free
0.169 0.167 0.199
Expression system: Escherichia coli BL21(DE3)

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