› 3sbc

X-ray diffraction
2.8Å resolution

Crystal structure of Saccharomyces cerevisiae TSA1C47S mutant protein

Released:
DOI: 10.2210/pdb3sbc/pdb
PDB entry 3sbc coloured by chain, front view.
PDB entry 3sbc coloured by chain, side view.
PDB entry 3sbc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Disulfide biochemistry in 2-cys peroxiredoxin: requirement of Glu50 and Arg146 for the reduction of yeast Tsa1 by thioredoxin.
Tairum CA, de Oliveira MA, Horta BB, Zara FJ, Netto LE
J Mol Biol 424 28-41 (2012)
PMID: 22985967

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxiredoxin TSA1 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 216 amino acids
Theoretical weight: 23.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P34760 (Residues: 1-196; Coverage: 100%)
Gene names: TPX1, TSA, TSA1, YML028W, ZRG14
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand DTU 5 x DTU
Ligand DTV 1 x DTV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: C2
Unit cell:
a: 239.983Å b: 51.961Å c: 192.355Å
α: 90° β: 92.33° γ: 90°
R-values:
R R work R free
0.205 0.202 0.274
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Peroxiredoxins: guardians against oxidative stress and modulators of peroxide signaling.
Perkins et al. (2015)
7 more

8 mentions without citation

Infinite Assembly of Folded Proteins in Evolution, Disease, and Engineering.
Garcia-Seisdedos et al. (2019)
7 more