› 3sbx

X-ray diffraction
2.5Å resolution

Crystal structure of a putative uncharacterized protein from Mycobacterium marinum bound to adenosine 5'-monophosphate AMP

Released:
DOI: 10.2210/pdb3sbx/pdb
PDB entry 3sbx coloured by chain, front view.
PDB entry 3sbx coloured by chain, side view.
PDB entry 3sbx coloured by chain, top view.
Source organism: Mycobacterium marinum M
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Reaction catalysed: 
N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate + H(2)O = N(6)-(dimethylallyl)adenine + D-ribose 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytokinin riboside 5'-monophosphate phosphoribohydrolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 189 amino acids
Theoretical weight: 20.16 KDa
Source organism: Mycobacterium marinum M
Expression system: Escherichia coli
UniProt:
  • Canonical: B2HS63 (Residues: 2-186; Coverage: 99%)
Gene name: MMAR_4233
Sequence domains: Possible lysine decarboxylase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand AMP 8 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P1
Unit cell:
a: 67.24Å b: 80.12Å c: 85.69Å
α: 106.7° β: 90.13° γ: 98.81°
R-values:
R R work R free
0.245 0.242 0.288
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more

6 mentions without citation

Structures of the calcium-activated, non-selective cation channel TRPM4.
Guo et al. (2017)
5 more