› 3sgu

X-ray diffraction
1.7Å resolution

Crystal structure of Methanothermobacter thermautotrophicus orotidine 5'-monophosphate decarboxylase covalently modified by 5-fluoro-6-iodo-UMP

Released:
DOI: 10.2210/pdb3sgu/pdb
PDB entry 3sgu coloured by chain, front view.
PDB entry 3sgu coloured by chain, side view.
PDB entry 3sgu coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Entry authors: Liu Y, Kotra LP, Pai EF

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate = UMP + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotidine 5'-phosphate decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 26.83 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O26232 (Residues: 1-228; Coverage: 100%)
Gene names: MTH_129, pyrF
Sequence domains: Orotidine 5'-phosphate decarboxylase / HUMPS family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand 5FU 1 x 5FU
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2221
Unit cell:
a: 57.891Å b: 103.709Å c: 73.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.196
Expression system: Escherichia coli

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