› 3sj8

X-ray diffraction
2.2Å resolution

Crystal structure of the 3C protease from coxsackievirus A16

Released:
DOI: 10.2210/pdb3sj8/pdb
PDB entry 3sj8 coloured by chain, front view.
PDB entry 3sj8 coloured by chain, side view.
PDB entry 3sj8 coloured by chain, top view.
Source organism: Coxsackievirus A16
Primary publication:
Enterovirus 71 and coxsackievirus A16 3C proteases: binding to rupintrivir and their substrates and anti-hand, foot, and mouth disease virus drug design.
Lu G, Qi J, Chen Z, Xu X, Gao F, Lin D, Qian W, Liu H, Jiang H, Yan J, Gao GF
J Virol 85 10319-31 (2011)
PMID: 21795339

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3C protease Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.09 KDa
Source organism: Coxsackievirus A16
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C8CIL7 (Residues: 1-183; Coverage: 28%)
Sequence domains: 3C cysteine protease (picornain 3C)
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43
Unit cell:
a: 40.586Å b: 40.586Å c: 99.287Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Enterovirus 71 infection and vaccines.
Yi et al. (2017)
17 more

3 mentions without citation

Enterovirus 71 and coxsackievirus A16 3C proteases: binding to rupintrivir and their substrates and anti-hand, foot, and mouth disease virus drug design.
Lu et al. (2011)
2 more