3sjb

X-ray diffraction
3.3Å resolution

Crystal structure of S. cerevisiae Get3 in the open state in complex with Get1 cytosolic domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-547080 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATPase GET3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 40.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12154 (Residues: 1-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Golgi to ER traffic protein 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 93 amino acids
Theoretical weight: 11.29 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53192 (Residues: 19-103; Coverage: 36%)
Gene names: GET1, MDM39, YGL020C
Sequence domains: CHD5-like protein
Structure domains: Helix hairpin bin

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6522
Unit cell:
a: 112.9Å b: 112.9Å c: 310.481Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.243
Expression system: Escherichia coli BL21(DE3)