› 3spx

X-ray diffraction
1.79Å resolution

Crystal structure of O-Acetyl Serine Sulfhydrylase from Leishmania donovani

Released:
DOI: 10.2210/pdb3spx/pdb
PDB entry 3spx coloured by chain, front view.
PDB entry 3spx coloured by chain, side view.
PDB entry 3spx coloured by chain, top view.
Source organism: Leishmania donovani
Primary publication:
The narrow active-site cleft of O-acetylserine sulfhydrylase from Leishmania donovani allows complex formation with serine acetyltransferases with a range of C-terminal sequences.
Raj I, Kumar S, Gourinath S
Acta Crystallogr D Biol Crystallogr 68 909-19 (2012)
PMID: 22868756

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124171 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase beta chain-like PALP domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 35.91 KDa
Source organism: Leishmania donovani
Expression system: Escherichia coli
UniProt:
  • Canonical: G1C2I2 (Residues: 1-325; Coverage: 100%)
Gene name: OASS
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand NA 1 x NA
Ligand PEG 1 x PEG
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21212
Unit cell:
a: 115.266Å b: 61.974Å c: 43.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The cysteine regulatory complex from plants and microbes: what was old is new again.
Jez et al. (2013)
1 more

2 mentions without citation

Insights into multifaceted activities of CysK for therapeutic interventions.
Joshi et al. (2019)
1 more