› 3ss8

X-ray diffraction
2.51Å resolution

Crystal structure of NFeoB from S. thermophilus bound to GDP.AlF4- and K+

Released:
DOI: 10.2210/pdb3ss8/pdb
PDB entry 3ss8 coloured by chain, front view.
PDB entry 3ss8 coloured by chain, side view.
PDB entry 3ss8 coloured by chain, top view.
Source organism: Streptococcus thermophilus LMG 18311
Primary publication:
The initiation of GTP hydrolysis by the G-domain of FeoB: insights from a transition-state complex structure.
Ash MR, Maher MJ, Guss JM, Jormakka M
OpenAccess logo PLoS One 6 e23355 (2011)
PMID: 21858085

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferrous iron transport protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 30.2 KDa
Source organism: Streptococcus thermophilus LMG 18311
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5M586 (Residues: 1-270; Coverage: 38%)
Gene names: feoB, stu0608
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GDP 2 x GDP
Ligand K 2 x K
Ligand MG 2 x MG
Ligand ALF 2 x ALF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 48.346Å b: 75.401Å c: 156.494Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.252
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial ferrous iron transport: the Feo system.
Lau et al. (2016)
3 more

6 mentions without citation

Ins and Outs: Recent Advancements in Membrane Protein-Mediated Prokaryotic Ferrous Iron Transport.
Brown et al. (2021)
5 more