› 3ste

X-ray diffraction
2.05Å resolution

Crystal structure of a mutant (Q202A) of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) from Neisseria meningitidis

Released:
DOI: 10.2210/pdb3ste/pdb
PDB entry 3ste coloured by chain, front view.
PDB entry 3ste coloured by chain, side view.
PDB entry 3ste coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Primary publication:
An extended β7α7 substrate-binding loop is essential for efficient catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase.
Allison TM, Hutton RD, Jiao W, Gloyne BJ, Nimmo EB, Jameson GB, Parker EJ
Biochemistry 50 9318-27 (2011)
PMID: 21942786

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 30.46 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JZ55 (Residues: 1-280; Coverage: 100%)
Gene names: NMB1283, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 82.002Å b: 85.709Å c: 163.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.247
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Exploring modular allostery via interchangeable regulatory domains.
Fan et al. (2018)
2 more

1 mention without citation

Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa.
Nelson et al. (2013)