› 3suw

X-ray diffraction
1.9Å resolution

Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with NHAc-CAS

Released:
DOI: 10.2210/pdb3suw/pdb
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PDB entry 3suw coloured by chain, top view.
Source organism: Paenibacillus sp. TS12
Primary publication:
Gaining insight into the inhibition of glycoside hydrolase family 20 exo-β-N-acetylhexosaminidases using a structural approach.
Sumida T, Stubbs KA, Ito M, Yokoyama S
Org Biomol Chem 10 2607-12 (2012)
PMID: 22367352

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112376 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-N-acetylhexosaminidase Chain: A
Molecule details ›
Chain: A
Length: 525 amino acids
Theoretical weight: 57.57 KDa
Source organism: Paenibacillus sp. TS12
Expression system: Escherichia coli
UniProt:
  • Canonical: D2KW09 (Residues: 1-502; Coverage: 51%)
Gene name: hex1t
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GC2 1 x GC2
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: C2221
Unit cell:
a: 95.735Å b: 101.988Å c: 107.852Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.148 0.195
Expression system: Escherichia coli

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Citations

1 review citation

α-Mannosidase and β-D-N-acetylhexosaminidase outside the wall: partner exoglycosidases involved in fruit ripening process.
Morales-Quintana et al. (2023)
18 other articles

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