› 3sw1

X-ray diffraction
2.63Å resolution

Structure of a full-length bacterial LOV protein

Released:
DOI: 10.2210/pdb3sw1/pdb
PDB entry 3sw1 coloured by chain, front view.
PDB entry 3sw1 coloured by chain, side view.
PDB entry 3sw1 coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Primary publication:
Structural basis for the slow dark recovery of a full-length LOV protein from Pseudomonas putida.
Circolone F, Granzin J, Jentzsch K, Drepper T, Jaeger KE, Willbold D, Krauss U, Batra-Safferling R
J Mol Biol 417 362-74 (2012)
PMID: 22326872

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensory box protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.62 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q88E39 (Residues: 1-142; Coverage: 100%)
Gene name: PP_4629
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P61
Unit cell:
a: 55.067Å b: 55.067Å c: 221.993Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Plant flavoprotein photoreceptors.
Christie et al. (2015)
3 more

10 mentions without citation

Photochemistry of flavoprotein light sensors.
Conrad et al. (2014)
9 more