› 3t2x

X-ray diffraction
1.15Å resolution

Structure of shwanavidin low affinity mutant (F43A)

Released:
DOI: 10.2210/pdb3t2x/pdb
PDB entry 3t2x coloured by chain, front view.
PDB entry 3t2x coloured by chain, side view.
PDB entry 3t2x coloured by chain, top view.
Source organism: Shewanella denitrificans OS217
Primary publication:
Structural adaptation of a thermostable biotin-binding protein in a psychrophilic environment.
Meir A, Bayer EA, Livnah O
J Biol Chem 287 17951-62 (2012)
PMID: 22493427

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin/streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 122 amino acids
Theoretical weight: 12.96 KDa
Source organism: Shewanella denitrificans OS217
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12QS6 (Residues: 43-162; Coverage: 70%)
Gene name: Sden_0912
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R3
Unit cell:
a: 113.933Å b: 113.933Å c: 83.431Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.145 0.171
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Streptavidin-biotin technology: improvements and innovations in chemical and biological applications.
Dundas et al. (2013)
1 more

1 mention without citation

Structural adaptation of a thermostable biotin-binding protein in a psychrophilic environment.
Meir et al. (2012)