› 3t6f

X-ray diffraction
1.22Å resolution

Biotin complex of Y54F core streptavidin

Released:
DOI: 10.2210/pdb3t6f/pdb
PDB entry 3t6f coloured by chain, front view.
PDB entry 3t6f coloured by chain, side view.
PDB entry 3t6f coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Second-contact shell mutation diminishes streptavidin-biotin binding affinity through transmitted effects on equilibrium dynamics.
Baugh L, Le Trong I, Cerutti DS, Mehta N, Gülich S, Stayton PS, Stenkamp RE, Lybrand TP
Biochemistry 51 597-607 (2012)
PMID: 22145986

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.27 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

4 bound ligands:
Ligand BTN 2 x BTN
Ligand BSO 2 x BSO
Ligand GOL 4 x GOL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I222
Unit cell:
a: 46.611Å b: 94.114Å c: 104.595Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.128 0.151
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Stabilizing biocatalysts.
Bommarius et al. (2013)
3 other articles

1 mention without citation

Second-contact shell mutation diminishes streptavidin-biotin binding affinity through transmitted effects on equilibrium dynamics.
Baugh et al. (2012)