› 3t7f

X-ray diffraction
1.89Å resolution

Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway

Released:
DOI: 10.2210/pdb3t7f/pdb
PDB entry 3t7f coloured by chain, front view.
PDB entry 3t7f coloured by chain, side view.
PDB entry 3t7f coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway.
Taherbhoy AM, Tait SW, Kaiser SE, Williams AH, Deng A, Nourse A, Hammel M, Kurinov I, Rock CO, Green DR, Schulman BA
Mol Cell 44 451-61 (2011)
PMID: 22055190

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like modifier-activating enzyme ATG7 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.56 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38862 (Residues: 1-289; Coverage: 46%)
Gene names: APG7, ATG7, CVT2, YHR171W
Sequence domains: Ubiquitin-like modifier-activating enzyme ATG7 N-terminus

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 56.856Å b: 74.677Å c: 76.047Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.197
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Autophagy modulation as a potential therapeutic target for diverse diseases.
Rubinsztein et al. (2012)
29 more

2 mentions without citation

Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway.
Taherbhoy et al. (2011)
1 more