3tcg

X-ray diffraction
2Å resolution

Crystal structure of E. coli OppA complexed with the tripeptide KGE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-150077 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Periplasmic oligopeptide-binding protein OppA Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 524 amino acids
Theoretical weight: 59.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P23843 (Residues: 27-543; Coverage: 100%)
Gene names: JW1235, b1243, oppA
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:
KGE Peptide Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 3 amino acids
Theoretical weight: 333 Da
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 62.487Å b: 201.308Å c: 206.947Å
α: 90° β: 95.57° γ: 90°
R-values:
R R work R free
0.202 0.199 0.245
Expression systems:
  • Escherichia coli BL21
  • Not provided