› 3tf6

X-ray diffraction
2.35Å resolution

Crystal structure of Neutrophil gelatinase-associated lipocalin (C87S mutant) in complex with Europium and the siderophore analog tren(cam)(1,2-hopo)2

Released:
DOI: 10.2210/pdb3tf6/pdb
PDB entry 3tf6 coloured by chain, front view.
PDB entry 3tf6 coloured by chain, side view.
PDB entry 3tf6 coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil gelatinase-associated lipocalin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 179 amino acids
Theoretical weight: 20.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P80188 (Residues: 21-198; Coverage: 100%)
Gene names: HNL, LCN2, NGAL
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

5 bound ligands:
Ligand GOL 4 x GOL
Ligand EU 3 x EU
Ligand DBH 3 x DBH
Ligand SO4 3 x SO4
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 114.09Å b: 114.09Å c: 118.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.216
Expression system: Escherichia coli BL21(DE3)

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