3tjz

X-ray diffraction
2.9Å resolution

Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-145649 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ADP-ribosylation factor 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 164 amino acids
Theoretical weight: 18.67 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P11076 (Residues: 18-181; Coverage: 91%)
Gene names: ARF1, D1244, YDL192W
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Coatomer subunit gamma-1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 355 amino acids
Theoretical weight: 39.38 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53620 (Residues: 1-355; Coverage: 41%)
Gene names: COPG, COPG1
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
Coatomer subunit zeta-1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 153 amino acids
Theoretical weight: 17.48 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35604 (Residues: 1-153; Coverage: 86%)
Gene names: COPZ, COPZ1
Sequence domains: Clathrin adaptor complex small chain
Structure domains: Beta-Lactamase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P42212
Unit cell:
a: 163.579Å b: 163.579Å c: 145.188Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.26
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda