› 3tl1

X-ray diffraction
1.8Å resolution

Crystal structure of the Streptomyces coelicolor WhiE ORFVI polyketide aromatase/cyclase

Released:
DOI: 10.2210/pdb3tl1/pdb
PDB entry 3tl1 coloured by chain, front view.
PDB entry 3tl1 coloured by chain, side view.
PDB entry 3tl1 coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Insight into the molecular basis of aromatic polyketide cyclization: crystal structure and in vitro characterization of WhiE-ORFVI.
Lee MY, Ames BD, Tsai SC
Biochemistry 51 3079-91 (2012)
PMID: 22432862

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative polyketide cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.22 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23154 (Residues: 1-159; Coverage: 100%)
Gene names: SC6G9.18, SCO5315
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

2 bound ligands:
Ligand JRO 2 x JRO
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 64.517Å b: 40.488Å c: 66.806Å
α: 90° β: 102.03° γ: 90°
R-values:
R R work R free
0.19 0.183 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Disruption of aromatase homeostasis as the cause of a multiplicity of ailments: A comprehensive review.
Patel S. (2017)
2 more

2 mentions without citation

Diversity and function of arthropod endosymbiont toxins.
Massey et al. (2022)
1 more