› 3tm0

X-ray diffraction
2.1Å resolution

Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type IIIa AMPPNP Butirosin A Complex

Released:
DOI: 10.2210/pdb3tm0/pdb
PDB entry 3tm0 coloured by chain, front view.
PDB entry 3tm0 coloured by chain, side view.
PDB entry 3tm0 coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structural basis of APH(3')-IIIa-mediated resistance to N1-substituted aminoglycoside antibiotics.
Fong DH, Berghuis AM
Antimicrob Agents Chemother 53 3049-55 (2009)
PMID: 19433564

Function and Biology Details

Reaction catalysed: 
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside 3'-phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 30.88 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3Y5 (Residues: 2-264; Coverage: 100%)
Gene name: aphA
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand B31 1 x B31
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P42212
Unit cell:
a: 80.112Å b: 80.112Å c: 110.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.179 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
3 more

2 mentions without citation

Strategies to overcome the action of aminoglycoside-modifying enzymes for treating resistant bacterial infections.
Labby et al. (2013)
1 more