› 3to7

X-ray diffraction
1.9Å resolution

Crystal structure of yeast Esa1 HAT domain bound to coenzyme A with active site lysine acetylated

Released:
DOI: 10.2210/pdb3to7/pdb
PDB entry 3to7 coloured by chain, front view.
PDB entry 3to7 coloured by chain, side view.
PDB entry 3to7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
MYST protein acetyltransferase activity requires active site lysine autoacetylation.
Yuan H, Rossetto D, Mellert H, Dang W, Srinivasan M, Johnson J, Hodawadekar S, Ding EC, Speicher K, Abshiru N, Perry R, Wu J, Yang C, Zheng YG, Speicher DW, Thibault P, Verreault A, Johnson FB, Berger SL, Sternglanz R, McMahon SB, Côté J, Marmorstein R
EMBO J 31 58-70 (2012)
PMID: 22020126

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone acetyltransferase ESA1 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 33.15 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08649 (Residues: 160-435; Coverage: 62%)
Gene names: ESA1, O5257, YOR244W
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 1 x COA
3 bound ligands:
Ligand CAD 1 x CAD
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: I4132
Unit cell:
a: 183.04Å b: 183.04Å c: 183.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.226
Expression system: Escherichia coli

Quick links

Citations

25 review citations

The growing landscape of lysine acetylation links metabolism and cell signalling.
Choudhary et al. (2014)
24 more

2 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
1 more