› 3u06

X-ray diffraction
2.35Å resolution

Crystal structure of the kinesin-14 NcdG347D

Released:
DOI: 10.2210/pdb3u06/pdb
PDB entry 3u06 coloured by chain, front view.
PDB entry 3u06 coloured by chain, side view.
PDB entry 3u06 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Neck-motor interactions trigger rotation of the kinesin stalk.
Liu HL, Pemble CW, Endow SA
OpenAccess logo Sci Rep 2 236 (2012)
PMID: 22355749

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein claret segregational Chains: A, B
Molecule details ›
Chains: A, B
Length: 412 amino acids
Theoretical weight: 46.79 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20480 (Residues: 293-700; Coverage: 58%)
Gene names: CA(ND), CG7831, ncd
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 1 x MG
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 160.539Å b: 67.094Å c: 94.369Å
α: 90° β: 98.91° γ: 90°
R-values:
R R work R free
0.217 0.216 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Structural basis of small molecule ATPase inhibition of a human mitotic kinesin motor protein.
Park et al. (2017)
3 more

2 mentions without citation

Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines.
Cochran JC. (2015)
1 more