› 3u0j

X-ray diffraction
2.7Å resolution

Crystal structure of ADP-ribosyltransferase HopU1 of Pseudomonas syringae pv. Tomato DC3000

Released:
DOI: 10.2210/pdb3u0j/pdb
PDB entry 3u0j coloured by chain, front view.
PDB entry 3u0j coloured by chain, side view.
PDB entry 3u0j coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Primary publication:
Structure function analysis of an ADP-ribosyltransferase type III effector and its RNA-binding target in plant immunity.
Jeong BR, Lin Y, Joe A, Guo M, Korneli C, Yang H, Wang P, Yu M, Cerny RL, Staiger D, Alfano JR, Xu Y
J Biol Chem 286 43272-81 (2011)
PMID: 22013065

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP ribosyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 30.58 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88A91 (Residues: 1-264; Coverage: 100%)
Gene names: PSPTO_0501, hopU1
Sequence domains: ADP-ribosyltransferase exoenzyme
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43
Unit cell:
a: 92.558Å b: 92.558Å c: 101.626Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.212
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Alternative splicing at the intersection of biological timing, development, and stress responses.
Staiger et al. (2013)
19 more

5 mentions without citation

Novel bacterial ADP-ribosylating toxins: structure and function.
Simon et al. (2014)
4 more