› 3ujm

X-ray diffraction
2.74Å resolution

Crystal structure of the NTF2-like domain of the Drosophila melanogaster Rasputin protein

Released:
DOI: 10.2210/pdb3ujm/pdb
PDB entry 3ujm coloured by chain, front view.
PDB entry 3ujm coloured by chain, side view.
PDB entry 3ujm coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster.
Vognsen T, Kristensen O
Biochem Biophys Res Commun 420 188-92 (2012)
PMID: 22414690

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194242 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rasputin, isoform G Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 13.88 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VFT4 (Residues: 15-130; Coverage: 17%)
Gene names: CG9412, Dmel\CG9412, Dmel_CG9412, Nts, RIN, Rin, cg9412, rasp, rin
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 39.2Å b: 80.38Å c: 39.2Å
α: 90° β: 92.6° γ: 90°
R-values:
R R work R free
0.199 0.196 0.242
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mechanistic insights into mammalian stress granule dynamics.
Panas et al. (2016)
2 more

2 mentions without citation

Crystal structures of the human G3BP1 NTF2-like domain visualize FxFG Nup repeat specificity.
Vognsen et al. (2013)
1 more