Function and Biology Details
Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Botulinum/Tetanus toxin, catalytic chain
- Clostridium neurotoxin, translocation domain
- Clostridium neurotoxin, receptor binding N-terminal
- Concanavalin A-like lectin/glucanase domain superfamily
- Nontoxic nonhaemagglutinin C-terminal
- Clostridium neurotoxin, receptor-binding C-terminal
- Kunitz inhibitor STI-like superfamily
- Clostridium neurotoxin, translocation
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144087 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Botulinum neurotoxin type A
Molecule details ›
Chain: A
Length: 1296 amino acids
Theoretical weight: 149.45 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 1296 amino acids
Theoretical weight: 149.45 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
- Canonical:
P0DPI1 (Residues: 1-1296; Coverage: 100%)
Sequence domains:
- Clostridial neurotoxin zinc protease
- Clostridium neurotoxin, Translocation domain
- Clostridium neurotoxin, N-terminal receptor binding
- Clostridium neurotoxin, C-terminal receptor binding
Non-toxic nonhemagglutinin type A
Molecule details ›
Chain: B
Length: 1196 amino acids
Theoretical weight: 138.45 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 1196 amino acids
Theoretical weight: 138.45 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
- Canonical: Q45914 (Residues: 1-1193; Coverage: 100%)
Sequence domains:
- Clostridial neurotoxin zinc protease
- Clostridium neurotoxin, N-terminal receptor binding
- Botulinum neurotoxin, helical domain
- Nontoxic nonhaemagglutinin C-terminal
