› 3v4u

X-ray diffraction
1.64Å resolution

Structure of a monoclonal antibody complexed with its MHC-I antigen

Released:
DOI: 10.2210/pdb3v4u/pdb
PDB entry 3v4u coloured by chain, front view.
PDB entry 3v4u coloured by chain, side view.
PDB entry 3v4u coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The peptide-receptive transition state of MHC class I molecules: insight from structure and molecular dynamics.
Mage MG, Dolan MA, Wang R, Boyd LF, Revilleza MJ, Robinson H, Natarajan K, Myers NB, Hansen TH, Margulies DH
J Immunol 189 1391-9 (2012)
PMID: 22753930

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-207553 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTI-MHC-I MONOCLONAL ANTIBODY, 64-3-7 H CHAIN Chain: H
Molecule details ›
Chain: H
Length: 216 amino acids
Theoretical weight: 23.16 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.97 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: A2NHM3 (Residues: 1-219; Coverage: 100%)
Gene names: Igk-C, Igkc
Sequence domains:
Structure domains: Immunoglobulins
H-2 class I histocompatibility antigen, L-D alpha chain Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.12 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01897 (Residues: 70-78; Coverage: 3%)
Gene name: H2-L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 196.14Å b: 40.643Å c: 59.069Å
α: 90° β: 103.07° γ: 90°
R-values:
R R work R free
0.215 0.213 0.25
Expression system: Not provided

Quick links

Citations

15 review citations

Major Histocompatibility Complex (MHC) Class I and MHC Class II Proteins: Conformational Plasticity in Antigen Presentation.
Wieczorek et al. (2017)
14 more

1 mention without citation

The peptide-receptive transition state of MHC class I molecules: insight from structure and molecular dynamics.
Mage et al. (2012)