3v64

X-ray diffraction
2.85Å resolution

Crystal Structure of agrin and LRP4

Released:
Source organism: Rattus norvegicus
Primary publication:
Structural basis of agrin-LRP4-MuSK signaling.
Genes Dev 26 247-58 (2012)
PMID: 22302937

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-150420 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Low-density lipoprotein receptor-related protein 4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 349 amino acids
Theoretical weight: 39.47 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9QYP1 (Residues: 396-737; Coverage: 18%)
Gene names: Lrp4, Megf7
Sequence domains: Low-density lipoprotein receptor repeat class B
Structure domains:
Agrin C-terminal 22 kDa fragment Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 20.88 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P25304 (Residues: 1759-1959; Coverage: 10%)
  • Best match: P25304-4 (Residues: 1759-1948)
Gene names: Agrin, Agrn
Sequence domains: Laminin G domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 99.46Å b: 106.07Å c: 112.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.272
Expression system: Spodoptera frugiperda