› 3vb1

X-ray diffraction
2Å resolution

Crystal Structure of Anopholes gambiae odorant binding protein 20 in open state

Released:
DOI: 10.2210/pdb3vb1/pdb
PDB entry 3vb1 coloured by chain, front view.
PDB entry 3vb1 coloured by chain, side view.
PDB entry 3vb1 coloured by chain, top view.
Source organism: Anopheles gambiae
Primary publication:
A novel mechanism of ligand binding and release in the odorant binding protein 20 from the malaria mosquito Anopheles gambiae.
Ziemba BP, Murphy EJ, Edlin HT, Jones DN
Protein Sci 22 11-21 (2013)
PMID: 23081820

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AGAP005208-PA Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.47 KDa
Source organism: Anopheles gambiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7Q9J3 (Residues: 24-142; Coverage: 84%)
Gene names: AgaP_AGAP005208, OBP20
Sequence domains: PBP/GOBP family
Structure domains: Pheromone/general odorant binding protein domain

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
1 modified residue:
Ligand SME 1 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 34.923Å b: 36.463Å c: 92.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.231 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

A look inside odorant-binding proteins in insect chemoreception.
Brito et al. (2016)
1 more

6 mentions without citation

Real-space refinement in PHENIX for cryo-EM and crystallography.
Afonine et al. (2018)
5 more