› 3vk1

X-ray diffraction
2.2Å resolution

Green-fluorescent variant of the non-fluorescent chromoprotein Rtms5

Released:
DOI: 10.2210/pdb3vk1/pdb
PDB entry 3vk1 coloured by chain, front view.
PDB entry 3vk1 coloured by chain, side view.
PDB entry 3vk1 coloured by chain, top view.
Source organism: Montipora efflorescens
Primary publication:
A green fluorescent protein containing a QFG tri-peptide chromophore: optical properties and X-ray crystal structure.
Battad JM, Traore DA, Byres E, Rossjohn J, Devenish RJ, Olsen S, Wilce MC, Prescott M
OpenAccess logo PLoS One 7 e47331 (2012)
PMID: 23071789

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like non-fluorescent chromoprotein Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.08 KDa
Source organism: Montipora efflorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P83690 (Residues: 1-221; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand IOD 1 x IOD
Ligand CL 3 x CL
1 modified residue:
Ligand QFG 1 x QFG

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P4222
Unit cell:
a: 93.12Å b: 93.12Å c: 76.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.242
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Cyan fluorescent proteins derived from mNeonGreen.
Zarowny et al. (2022)

3 mentions without citation

A green fluorescent protein containing a QFG tri-peptide chromophore: optical properties and X-ray crystal structure.
Battad et al. (2012)
2 more