› 3vol

X-ray diffraction
2.4Å resolution

X-ray Crystal Structure of PAS-HAMP Aer2 in the CN-bound Form

Released:
DOI: 10.2210/pdb3vol/pdb
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PDB entry 3vol coloured by chain, front view.
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PDB entry 3vol coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginosa.
Sawai H, Sugimoto H, Shiro Y, Ishikawa H, Mizutani Y, Aono S
Chem Commun (Camb) 48 6523-5 (2012)
PMID: 22622145

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein McpB Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 25.8 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I6V6 (Residues: 173-384; Coverage: 31%)
Gene names: PA0176, aer-2, aer2, mcpB, tlpG
Sequence domains:
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand CYN 1 x CYN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P6522
Unit cell:
a: 83.02Å b: 83.02Å c: 107.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.223 0.268
Expression system: Escherichia coli

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Citations

3 review citations

Bacterial diguanylate cyclases: structure, function and mechanism in exopolysaccharide biofilm development.
Whiteley et al. (2015)
2 more

14 mentions without citation

Sensory Repertoire of Bacterial Chemoreceptors.
Ortega et al. (2017)
13 more

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