› 3vpq

X-ray diffraction
1.7Å resolution

Crystal structure of Bombyx mori sigma-class glutathione transferase in complex with glutathione

Released:
DOI: 10.2210/pdb3vpq/pdb
PDB entry 3vpq coloured by chain, front view.
PDB entry 3vpq coloured by chain, side view.
PDB entry 3vpq coloured by chain, top view.
Source organism: Bombyx mori
Primary publication:
Crystal structure of a Bombyx mori sigma-class glutathione transferase exhibiting prostaglandin E synthase activity.
Yamamoto K, Higashiura A, Suzuki M, Aritake K, Urade Y, Uodome N, Nakagawa A
Biochim Biophys Acta 1830 3711-8 (2013)
PMID: 23458683

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutathione transferase Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.37 KDa
Source organism: Bombyx mori
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5CCJ4 (Residues: 1-204; Coverage: 100%)
Gene names: GST sigma, GSTs2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
3 bound ligands:
Ligand PGO 10 x PGO
Ligand PEG 1 x PEG
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P6522
Unit cell:
a: 56.081Å b: 56.081Å c: 209.244Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.227
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Draft Genome of the Scarab Beetle Oryctes borbonicus on La Réunion Island.
Meyer et al. (2016)
10 more

2 mentions without citation

Comparative analyses and structural insights of new class glutathione transferases in Cryptosporidium species.
Mfeka et al. (2020)
1 more