› 3vtt

X-ray diffraction
1.7Å resolution

High Resolution crystal structure of Dengue 3 Envelope protein domain III (ED3)

Released:
DOI: 10.2210/pdb3vtt/pdb
PDB entry 3vtt coloured by chain, front view.
PDB entry 3vtt coloured by chain, side view.
PDB entry 3vtt coloured by chain, top view.
Source organism: Dengue virus 3 Philippines/H87/1956
Primary publication:
High resolution crystal structure of dengue-3 envelope protein domain III suggests possible molecular mechanisms for serospecific antibody recognition.
Elahi M, Islam MM, Noguchi K, Yohda M, Kuroda Y
Proteins 81 1090-5 (2013)
PMID: 23239402

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151098 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Envelope protein E Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 11.62 KDa
Source organism: Dengue virus 3 Philippines/H87/1956
Expression system: Escherichia coli
UniProt:
  • Canonical: P27915 (Residues: 574-678; Coverage: 3%)
Sequence domains: Flavivirus glycoprotein, immunoglobulin-like domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P21
Unit cell:
a: 51.03Å b: 37.68Å c: 52.54Å
α: 90° β: 105.03° γ: 90°
R-values:
R R work R free
0.208 0.206 0.255
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Cross-reactivities between human IgMs and the four serotypes of dengue virus as probed with artificial homodimers of domain-III from the envelope proteins.
Zidane et al. (2013)
8 more

7 mentions without citation

The mechanism of differential neutralization of dengue serotype 3 strains by monoclonal antibody 8A1.
Zhou et al. (2013)
6 more